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Panels › Saliva Panels › Estradiol


Estradiol is the most biologically active form of all the naturally occurring estrogens and the most important one. In females, during reproductive years, it is largely is produced by the ovaries. Smaller amounts are also produced by the adrenal glands and from metabolism of testosterone in the peripheral circulation. Estradiol is responsible for development of sex organs and characteristics in females. It is also found in males, but in much smaller concentrations.
Estradiol circulates mostly bound to estrogen binding globulin (also referred to as SHBG) with a smaller fraction loosely bound to albumin. A small percentage circulates unbound to proteins (free form). The free and weakly bound are considered biologically active forms. Estradiol levels are most commonly used to evaluate ovarian function, and in males to diagnose the cause of gynecomastia. Estradiol is also used to monitor menopausal hormone replacement therapy.

Determination of Estradiol is performed by Enzyme Immunoassay.

Patient Preparation
Avoid eating, brushing prior to sample collection. At time of collection, briefly rinse mouth with water and begin collection. Do not collect specimen if there is damage to gums, mouth or there are signs of bleeding where blood contamination will occur, as these will completely invalidate the test. Morning specimen is preferred. Where possible/appropriate, avoid medication that can alter levels (at least 48 hrs prior to specimen collection).

Specimen requirements
Collect blood specimen into plain or redtop evacuated tube. Centrifuge and separate serum from cells (plain tube). Volume requested: at least 1 mL serum. Minimum volume: 0.5 mL

Shipping Instructions
Ship specimen at ambient temperatures. Ensure cap on the tube is on reasonably tight to prevent leaking/spillage.

Additional information