Saliva Tests (TOS)
Salivary Hormones
Steroid Hormones
Androstenedione
Cortisol
DHEA
DHEA-S
Estriol
Estradiol
17-OH progesterone
Progesterone
Testosterone
 

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Tests › Saliva tests › Salivary Hormones › Steroid Hormones › Androstenedione

Androstenedione

Significance
Androstenedione is a weak androgen derived from DHEA and 17-hydroxy progesterone. It is synthesized in the adrenal glands and the gonads, and serves as an important precursor for synthesis of testosterone and Estradiol. It is under ACTH regulation, and secreted synchronously with Cortisol. Therefore, highest values occur early morning and lowest around midnight. In females, levels also vary with the menstrual cycle with higher levels at mid-cycle. Levels decline with age beginning at ages 25-30. Androstenedione is converted to Testosterone and Dihydrotestosterone in peripheral tissues (hair follicles, the sebaceous glands, the prostate and the external genitalia). In men, the contribution to testosterone levels from Androstenedione is minimal; whereas in women, it is substantial with regards to source for the total androgen production and the effects. Androstenedione is elevated in congenital adrenal hyperplasia with 21-hydroxylase deficiency, in 60% of hirsuite women, polycystic ovary syndrome, Cushing’s syndrome, and ectopic ACTH-producing tumors. Androstenedione determinations are primarily used for investigation of females presenting with hyperandrogenic symptoms (hirsuitism, acne, virilization) and for monitoring/managing antiandrogenic treatment. Salivary levels correlate well with plasma concentrations. Studies have demonstrated salivary androstenedione determinations to be reliable and suitable for investigating hyper-androgenic females and monitoring their treatment.

Analysis
Determination of Androstenedione is performed by enzyme immunoassay.

Patient Preparation
Avoid eating, brushing prior to sample collection. At time of collection, briefly rinse mouth with water and begin collection. Do not collect specimen if there is damage to gums, mouth or there are signs of bleeding where blood contamination will occur, as these will completely invalidate the test. Morning specimen is preferred. Where possible/appropriate, avoid medication that can alter levels (at least 48 hrs prior to specimen collection).

Specimen requirements
Saliva specimen – Collect saliva directly into container provided. Collect at least 5 mL but 10 mL would be preferred.

Shipping Instructions
Ship specimen at ambient temperatures. Ensure cap on the tube is on reasonably tight to prevent leaking/spillage.

Additional information