Saliva Tests (TOS)
Salivary Hormones
Steroid Hormones
Androstenedione
Cortisol
DHEA
DHEA-S
Estriol
Estradiol
17-OH progesterone
Progesterone
Testosterone
 

TOS - To be offered shortly


Tests › Saliva tests › Salivary Hormones › Steroid Hormones › Testosterone

Testosterone

Significance
Testosterone is the major androgen responsible for sexual differentiation and male secondary sex characteristics. In the circulation, approximately 55% is tightly bound to sex hormone binding globulin (SHBG), 42% to albumin, and about 3% is free (unbound). The free and about half of the albumin fraction is bioactive. In males, testosterone is primarily secreted by the testes and levels exhibit diurnal variation with the highest levels between 6 and 9 AM and lowest around midnight. Testosterone levels begin to decline with age starting at about 40 yrs. In females, about half of the testosterone is derived from peripheral conversion of Androstenedione. Major applications of testosterone measurement reside with assessment of sexual development, gonadal disorders, hyperandrogenic syndromes, menstrual disorders, and hormone replacement therapy. The pattern of salivary testosterone mirrors that of plasma, and levels correlate very well with the free plasma fraction. Salivary assessment has been shown to be useful with good discriminating power in evaluating male hypogonadism, polycystic ovary disease, Idiopathic hirsuitism, hormone replacement therapy, monitoring anti-androgenic therapy, and in conditions where SHBG levels are altered (i.e. hyper/hypothyroidism).

Analysis
Determination of Cortisol is performed by enzyme immunoassay.

Patient Preparation
Avoid eating, brushing just before sample collection. At time of collection, briefly rinse mouth with water and begin collection. Do not collect specimen if there is damage to gums, mouth or there are signs of bleeding where blood contamination will occur, as these will completely invalidate the test. Morning specimen is preferred. Where possible/appropriate avoid medication that can alter testosterone levels (at least 48 hrs prior to specimen collection).

Specimen requirements
Saliva specimen – Collect saliva directly into container provided. Collect at least 5 mL but 10 mL would be preferred.

Shipping Instructions
Ship specimen at ambient temperatures. Ensure cap on the tube is on reasonably tight to prevent leaking/spillage.

Additional information
http://www.labtestsonline.org/understanding/analytes/testosterone/test.html